Not known Facts About attune flow cytometer

Not known Facts About attune flow cytometer

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Info-enriched characterization of mobile populations. Morphological info from pictures can add for the richness of flow cytometry knowledge. For example, the figure reveals an if not standard apoptosis assay working with Annexin V and PI, incorporating cell imaging to characterize cells in Every single inhabitants to expose morphologically distinctive attributes. These insights could not have been gained from multiplex staining by itself.

No matter if you’re a starter or an expert, discover our resources to progress your analysis and expertise in flow cytometry.

Combine acoustic concentrating technological innovation with substantial-parameter Examination for flexible spectral unmixing and standard workflows.

Find out new insights into your experimental info by visualizing populations employing merged fluorescence and morphology data. Our application interface wizards allow downsampling, concatenation (merged information) and dimensionality tools that happen to be constructed in to the program for an intuitive, streamlined info Assessment working experience.

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Listed here, an experienced user has gated singlets confidently. Following evaluating the handbook singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate is made up of in excess of four% aggregates.

Optimizing gating approach. Even robust handbook singlet gating is error-susceptible and stays a subjective final decision stage in Pretty much all flow cytometry assays. Imaging may be used to substantiate and adjust gates more info to incorporate only single cells of desire.

Dependable image quality even at high throughput Acoustic concentrating as well as a large-speed digicam Merge to image these T cells constantly at small or substantial flow rates. Conveniently alter focus and camera options to meet experimental prerequisites.

Early indications of this contamination had originally been dismissed as particles. Backgating to find out new populations. Backgating imaged cells to the Attune CytPix Flow Cytometer also helps you to use morphological attributes to find appealing subpopulations that could not be obvious from flow cytometry info alone.

Potentially most of all, these functions consist of cells of clearly various phenotypes which could lead on to incorrect conclusions pertaining to double good activities (specifically in uncommon populations).

AI-pushed automatic picture Evaluation While using the Attune Cytometric Software program interprets celebration functions into distinct morphology parameters that may be combined with common fluorescence and scatter parameters to expose highly reproducible population changes and distinctive subsets.

Application Observe: Automated graphic analysis cuts down consumer-to-person variability in flow cytometry gating methods

Designed-in dimensionality reduction equipment increased resolution into populations. Fluorescently gated populations back again-gated on to UMAP plot to verify expected populace resolution in lysed full human blood. User can see gatherings from Each and every cluster for visual confirmation of mobile phenotypes.

No wash/no lyse leukocyte Evaluation with imaging. Cells had been obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Expert UX/UI permits customers to prepare AI types for expanded applications. (A) The “Coach Design” feature offers annotation tools and true-time model coaching precision.

At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing technologies, which utilizes audio waves to meticulously align cells inside the flow cell. This progressive tactic surpasses traditional hydrodynamic techniques, causing more quickly processing speeds, Remarkable facts top quality, and greater sample throughput.

The Attune Xenith cytometer is often a substantial-parameter spectral analyzer, providing each spectral unmixing and traditional flow abilities for extensive and adaptable mobile analysis.

Agilent Value Promise guarantees you at the very least a decade of instrument use with the date you purchase. If not, We are going to credit history you with the residual value of the method towards an upgraded design.

What seemed to be a homogeneous population is in fact far more diverse—an Perception that ought to be regarded as when interpreting effects.